Pensacola Blue Wahoos

The franchise began in 1959 as the Charleston White Sox of the South Atlantic League (now the Southern League); it subsequently moved several times, playing in Charleston, South Carolina, (1959–1961); Savannah, Georgia, (1962); Lynchburg, Virginia, (1963–1965); and Evansville, Indiana, (1966–1968), before moving to Columbus, Georgia, in 1969 to play in Golden Park. The team was known as the Columbus Astros from 1970 to 1988, when it became an affiliate of the Houston Astros. Following the 1988 season, new owner Steve Bryant held a contest among season ticket holders to rename the team, and as a result, in 1989 the team became the Columbus Mudcats. In 1991, Bryant moved the club to Zebulon, North Carolina, and renamed them the Carolina Mudcats.

In Zebulon, the team played in Five County Stadium and won Southern League championships in 1995 and 2003. In 2010, Quint Studer, owner of the Pensacola Pelicans independent baseball team, acquired the Mudcats franchise in a complicated series of moves and purchases designed to bring affiliated Double-A baseball to Pensacola. First, he sold the Pelicans to a group in Amarillo, Texas, where they became the Amarillo Sox in 2011. Studer then bought the Carolina Mudcats franchise. To settle this purchase, he had to facilitate the move of the Kinston Indians to Zebulon, where they assumed the Carolina Mudcats name and branding. Additionally, as Pensacola is within the franchise territory of the Mobile Bay Bears, Studer paid that team an undisclosed sum of money. The arrangements cost a total of $2 million.

The Blue Wahoos nickname was decided in a fan contest run by Wendy's in conjunction with the Pensacola News Journal. It refers to the wahoo (Acanthocybium solandri), a local fish species. Their team colors are Neon red, Gulf Coast royal, Blue Angel navy, and Tin Roof tin. It was officially announced on May 23, followed by the logo and colors on November 18.

Following the inaugural season that saw Billy Hamilton break the all-time professional baseball single season stolen base record, the team was named the Southern League's Organization of the Year. Two-time Masters Champion Bubba Watson became co-owner in January 2015.

In 2016, the Blue Wahoos were chosen by Baseball America as the Double-A winner of the prestigious Bob Freitas Award as the best overall franchise among the nation's 30 affiliates at this level. It was announced at the Baseball Winter Meetings at the Gaylord Resort and Convention Center in National Harbor, Maryland, just outside of Washington, D.C.

They entered into a two-year PDC with the Minnesota Twins that ran from 2019 to 2020. The start of the 2020 season was postponed due to the COVID-19 pandemic before ultimately being cancelled on June 30.

Starting in 2021, the Blue Wahoos were affiliated with the Miami Marlins. In a further result of Major League Baseball's restructuring of Minor League Baseball in 2021, the Blue Wahoos were organized into the eight-team Double-A South. In 2022, the Double-A South became known as the Southern League, the name historically used by the regional circuit prior to the 2021 reorganization.